Hannah Rigoni Explores Cave and Aquifer Dwelling Organisms
Hannah Rigoni Explores Cave and Aquifer Dwelling Organisms
PhD student Hannah Rigoni and her advisor, Professor Annette Engel, are working to shed light on cave and aquifer dwelling organisms and their interactions with groundwater geochemistry and microbial communities.
Karst groundwater aquifers provide drinking water to billions of people worldwide, but they are vulnerable to pollution. Aquifers are home to stygobionts—groundwater dwelling fauna—that aid in maintaining water quality by consuming microbial and surface-derived organic matter. Microbial communities in aquifers depend on both surface-derived organic nutrients and inorganic nutrients from water-rock interactions. Despite the global importance of karst aquifers, the relationship between stygobiont communities to groundwater geochemistry and microbial diversity, and their possible utility as water quality indicators, is unknown.
With two highly biodiverse study sites, the Dinaric Karst in Croatia and the Edwards Aquifer in Texas, Rigoni is using geochemical and molecular biological sequencing techniques (including amplicon sequencing, lipidomics, and metagenomics) to characterize groundwater chemistry, microbial community diversity and metabolic processes across these spatially extensive aquifers. These methods provide a foundation to characterize the food-web interactions between stygobionts and microbial communities.
Rigoni has so far uncovered microbial metabolic processes tied to nitrogen, carbon, and methane cycling in both aquifers. In the Dinaric Karst, she has quantified the contribution of microbial biomass produced from ammonia- and methane-oxidizers to stygobiont diets. With as much as 50% of the stygobionts food coming from microbial sources, Rigoni’s research emphasizes the importance of prioritizing water quality maintenance to ensure the survival of endangered stygobionts and the microbial communities on which they rely.
This research makes a scientific case for stygobiont conservation and management of groundwater resources that are an essential resource for many people across the world.